Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite.
نویسندگان
چکیده
Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Phenylboronic acid (PBA), benzoboroxole (BBA) and 2-(N,N-dimethylaminomethyl)phenylboronic acid (NBA) were employed to bind with ARS to form the complex probes. In particular, the ARS-NBA system with a high binding affinity can preferably react with peroxynitrite over hydrogen peroxide and other ROS/RNS due to the protection of the boron via the solvent-insertion B-N interaction. Our simple system produces a visible colorimetric change and on-off fluorescence response towards peroxynitrite. By coupling a chemical reaction that leads to an indicator displacement, we have developed a new sensing strategy, referred to herein as RIA (Reaction-based Indicator displacement Assay).
منابع مشابه
Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c4sc03983a Click here for additional data file.
Department of Chemistry, University of Bat bath.ac.uk; [email protected] School of Chemistry, University of Birm Midlands, B15 2TT, UK School of Pharmacy, East China University 130, Shanghai 200237, China Department of Chemistry and Biochemistry, Texas, 78712, USA. E-mail: anslyn@austin. CEITEC, Masaryk University, Kamenice 5, 6 † Electronic supplementary informa 10.1039/c4sc03983a Cite this:...
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عنوان ژورنال:
- Chemical science
دوره 6 5 شماره
صفحات -
تاریخ انتشار 2015